Journal
/
Biologia
/
CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

DOI:
10.3791/55526-v

•

07:37 min

•

June 25, 2017

•

, ,
1Centro de Ciencias Genómicas,Universidad Nacional Autónoma de México, 2Oncomedic Incorporation, Ciudad de México, 3Laboratorio Internacional de Investigación sobre el Genoma Humano,Universidad Nacional Autónoma de México

Capítulos

  • 00:05Título
  • 01:22CAPRRESI Oligonucleotide Design
  • 03:29Plasmid Disruption by PCR Followed by Restriction Enzyme Digestion
  • 05:15Plasmid Recovery Using DNA Ligation and Bacterial Transformation
  • 06:43Results: Example Sequence of Assembled Gene
  • 07:18Conclusion

Summary

Tadução automática

Tadução automática

Here, we present chimera assembly by plasmid recovery and restriction enzyme site insertion (CAPRRESI), a protocol based on the insertion of restriction enzyme sites into synonym DNA sequences and functional plasmid recovery. This protocol is a fast and low-cost method for fusing protein-coding genes.

Tags

CAPRRESIChimera AssemblyPlasmid RecoveryRestriction Enzyme Site InsertionGene FusionBiologia MolecularRpoDSigAAmpicillin ResistancePCRPerl ScriptsAmino Acid Sequence AlignmentSynonym DNARestriction Enzyme Sites

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Principles of Site-Specific Recombinase (SSR) Technology

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

High-throughput Yeast Plasmid Overexpression Screen

Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Read Article