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In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
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In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues

In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues

DOI:
10.3791/55529-v

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10:36 min

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March 21, 2017

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, , , , ,
1MTA-SE Pathobiochemistry Research Group,Semmelweis University, 2Department of Medical Chemistry, Molecular Biology and Pathobiochemistry,Semmelweis University, 3MTA-ELTE Research Group of Peptide Chemistry,Eötvös Loránd University

Capítulos

  • 00:05Título
  • 01:07GnRH Uptake
  • 02:39Type I GnRH Receptor Expression
  • 05:39Fluorescence Microscopy Imaging
  • 06:26FACS-assisted GnRH Quantification
  • 08:00Results: GnRH I Uptake and Type I GnRH Receptor Expression in Pharynx Carcinoma Cells
  • 09:25Conclusion

Summary

Tadução automática

Tadução automática

Selectively labeled fluorescent GnRH-I, -II and -III derivatives are reliable tools for tracking and quantifying their cellular uptake. This manuscript introduces experiments to visualize, quantify and compare the uptake efficiency of these GnRH conjugates in various cell lines.

Tags

In Vitro ImagingQuantificationDrug Targeting EfficiencyFluorescently Labeled GnRH AnaloguesHuman Cancer Cell CulturesTargeted Anti-cancer TherapyGnRH ReceptorsConfocal MicroscopyFlow CytometryGnRH FITCPreheated Treating MediaFixing SolutionFluorescent ProbeMounting Medium

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