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Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

DOI:
10.3791/55534-v

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11:12 min

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April 12, 2017

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, , , ,
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences, 2Tobacco Research Institute,Chinese Academy of Agricultural Sciences, 3Crop and Environment Sciences,Harper Adams University

Capítulos

  • 00:05Título
  • 00:40Construction of the HaDV2-free Cotton Bollworm Colony
  • 02:02Preparation of HaDV2-containing Liquid Fluids
  • 03:28Construction of the HaDV2-infected Cotton Bollworm Colony
  • 04:57Tissue Distribution of HaDV2
  • 07:01Bioassays Testing the Effect of HaDV2 on Host Development
  • 09:36Results: Characterization of Effects of Densovirus on Bollworms
  • 10:44Conclusion

Summary

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Here, we present a protocol to investigate the host-tissue distribution, transmission mode, and effect on the host fitness of a densovirus within a lepidopteran species, the cotton bollworm. This protocol can also be used for studying the interaction between other orally-transmitted viruses and their insect hosts.

Tags

Keywords: Cotton BollwormDensovirusHaDV2Virus-host InteractionVirus TransmissionHost FitnessPCRGenetic BackgroundSingle-pair MatingAdult MothsEgg CollectionLarval RearingVirus DetectionVirus-positive StrainVirus-negative Strain

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