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激光局部补骨脂素加合物的单分子分析

JoVE Journal
Genética

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JoVE Journal Genética

Single Molecule Analysis of Laser Localized Psoralen Adducts

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激光局部补骨脂素加合物的单分子分析

DOI:

10.3791/55541-v

•

11:46 min

•

April 20, 2017

•

Jing Huang, Himabindu Gali, Julia Gichimu, Marina A. Bellani, Durga Pokharel, Manikandan Paramasivam, Michael M. Seidman

¹Laboratory of Molecular Gerontology,National Institute on Aging, National Institutes of Health

Capítulos

00:05Título
00:53Calibrating the Targeting Laser
04:54Laser Localized Dig-TMP ICLs
07:44Harvesting Cells and Stretching DNA Fibers
09:51Results: Laser Localized Psoralen Adducts Reveal the DNA Damage Response to ICLs
11:21Conclusion

Summary

Tadução automática

激光器在对DNA损伤的细胞应答的研究经常使用的。然而，它们产生的病变，其间隔，频率，和碰撞与复制叉很少表征。在这里，我们描述了一种方法，使这些参数与激光局部间交联的决心。

Tags

Single Molecule Analysis Laser Localized Psoralen Adducts DNA Interstrand Crosslinks DNA Repair Laser Activated Compound Mirror Slide Calibration Laser Targeting Biological Calibration Trimethylpsoralen GFP Tagged Repair Factor U2OS Cell Line

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