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An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production
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Biologia
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JoVE Journal Biologia
An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

DOI:
10.3791/55544-v

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07:46 min

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March 27, 2017

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, ,
1Division of Cancer Biology and Genetics,Queen’s University Cancer Research Institute, 2Department of Pathology and Molecular Medicine,Queen’s University

Capítulos

  • 00:05Título
  • 00:40Spheroid Production by Cell Aggregation
  • 03:44Example Application: Embedding Spheroids in Collagen
  • 05:30Results: Representative Spheroid Formation
  • 06:54Conclusion

Summary

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Here, we describe a rapid and flexible protocol for the formation of 3D cell spheroids through cell aggregation. This is easily adapted to multiple cell types and is suitable for use in a variety of applications including cell migration, invasion, or anoikis assays, and for imaging and quantifying cell-matrix interactions.

Tags

Keywords: 3D Spheroid ProductionCell AggregationMethylcelluloseCell SuspensionCell CultureSerumTrypsin-EDTACell PelletCentrifugationCell CountingCell AssaysCell-cell InteractionsCell-matrix Interactions

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