Utilisation d'une ligne centrale pour les fluides veineuses, les drogues et l'administration des nutriments dans un modèle de souris de maladies graves

This protocol describes a centrally catheterized mouse model of prolonged critical illness. We combine the cecal ligation and puncture method to induce sepsis with the use of a central venous line for fluids, drugs and nutrient administration to mimic the human clinical setting.