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गंभीर बीमारी के एक माउस मॉडल में तरल पदार्थ, औषधि और पोषक तत्व प्रशासन के लिए एक केंद्रीय शिरापरक लाइन का प्रयोग करें
JoVE Journal
Medicina
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JoVE Journal Medicina
Use of a Central Venous Line for Fluids, Drugs and Nutrient Administration in a Mouse Model of Critical Illness
DOI:

09:17 min

May 02, 2017

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Capítulos

  • 00:05Título
  • 00:54Venous Line Preparation and Chronic Indwelling Catheter Placement
  • 05:06Cecal Ligation and Puncture (CLP)
  • 07:06Results: Representative Survival Curves after 5 or 7 Days of Critical Illness
  • 08:08Conclusion

Summary

Tadução automática

This protocol describes a centrally catheterized mouse model of prolonged critical illness. We combine the cecal ligation and puncture method to induce sepsis with the use of a central venous line for fluids, drugs and nutrient administration to mimic the human clinical setting.

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