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对于代谢物分析基于法 - 串联液相色谱 - 质谱法<em>金黄色葡萄球菌</em

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Bioquímica

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JoVE Journal Bioquímica

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

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对于代谢物分析基于法 - 串联液相色谱 - 质谱法<em>金黄色葡萄球菌</em

DOI:

10.3791/55558-v

•

08:03 min

•

March 28, 2017

•

David J. Samuels, Zhe Wang, Kyu Y. Rhee³, Shaun R. Brinsmade

¹Department of Biology,Georgetown University, ²Division of Infectious Diseases,Weill Cornell Medical College, ³Department of Medicine,Weill Cornell Medical College

Capítulos

00:05Título
00:50Establishment of Steady-state S. aureus Growth
02:39Sample Collection Setup and Sample Harvest
04:54Metabolite Extraction
05:54Results: Comparison of Wild Type S. aureus Cells to an Isogenic Mutant Lacking the Global Transcriptional Regulator, CodY
07:20Conclusion

Summary

Tadução automática

在这里，我们描述了用于通过液相色谱法和质谱分析法从金黄色葡萄球菌和它们的后续分析的代谢物的提取的协议。

Tags

Keywords: Tandem Liquid Chromatography-Mass Spectrometry Metabolite Analysis Staphylococcus Aureus Metabolism Pathogenesis Nutrient Depletion Host Infection Optical Density Extraction Buffer Quenching Solution

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