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Journal / Developmental Biology / 5-mC点印迹测定定量软骨细胞去分化的DNA甲基化水平…
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5-mC点印迹测定定量软骨细胞去分化的DNA甲基化水平
 
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5-mC点印迹测定定量软骨细胞去分化的DNA甲基化水平

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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我们提出了一种基于5-甲基胞嘧啶（5-mC）斑点印迹定量DNA甲基化的方法。我们在软骨细胞去分化中测定了5-mC的水平。这种简单的技术可用于快速测定ACI治疗中的软骨细胞表型。

Tags

发育生物学，第123期，细胞培养，单层扩增，软骨细胞去分化，DNA甲基化，5-mC斑点印迹分析，基因组DNA提取
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