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5-एमसी डॉट ब्लाट परख डीएनए मेथिलिकेशन लेवल ऑफ़ चॉन्ड्रोसाइट डिडिएफेनिफिकेशन<em> विट्रो में</em
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1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics,Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry,The Chinese University of Hong Kong, 4School of Biomedical Engineering,Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center,Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College
Capítulos
- 00:05Título
- 01:52Human Articular Cartilage Tissue Collection and Chondrocyte Culture
- 03:33Genomic DNA Extraction
- 05:47Dot Blot nalysis
- 07:55Results: DNA Methylation Increases During Chondrocyte Dedifferentiation
- 08:45Conclusion
हम 5-मेथिलसीटोसिन (5-एमसी) डॉट ब्लोट के आधार पर डीएनए मेथिलिकेशन को मापने के लिए एक विधि प्रस्तुत करते हैं। हमने chondrocyte dedifferentiation के दौरान 5-एमसी स्तर निर्धारित किया था। एसीआई उपचार में chondrocyte phenotype को शीघ्रता से निर्धारित करने के लिए यह सरल तकनीक का इस्तेमाल किया जा सकता है।
