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重组蛋白表达，结晶和生物物理研究<em>芽孢杆菌</em> - 保守的核苷酸焦磷酸化酶，BcMazG

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Bioquímica

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JoVE Journal Bioquímica

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

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重组蛋白表达，结晶和生物物理研究<em>芽孢杆菌</em> - 保守的核苷酸焦磷酸化酶，BcMazG

DOI:

10.3791/55576-v

•

12:23 min

•

May 16, 2017

•

Meong Il Kim, Choongdeok Lee, Minsun Hong

¹Division of Biological Science and Technology,Yonsei University

Capítulos

00:05Título
00:55Recombinant Protein Production and Purification of rBC1531
04:47Removal of Affinity Tags by Thrombin Proteolysis
06:32Crystallization Screening and Optimization of rBC1531
07:30Colorimetric NTPase Assay
08:33Results: Purification, Characterization, and Crystallization of rBC1531
10:31Conclusion

Summary

Tadução automática

炭疽芽孢杆菌是致命性吸入性炭疽的专性病原体，因此对其基因和蛋白质的研究受到严格管制。另一种方法是研究直系同源基因。我们描述了蜡状芽孢杆菌Bc1531中的炭疽杆菌直系同源物的生物物理化学研究，需要最少的实验设备和缺乏严重的安全性问题。

Tags

Recombinant Protein Bacillus Nucleotide Pyrophosphorylase BcMazG Protein Expression Protein Crystallization Biophysical Studies E. Coli Chemically Competent Cells Transformation Plasmid DNA DNA Sequencing Protein Purification IPTG Induction Cell Lysis Protein Characterization

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