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多层皮质Ca<sup> 2+</sup>使用棱镜探针和小型荧光显微镜成像自由移动的小鼠
JoVE Journal
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JoVE Journal Comportamento
Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy
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多层皮质Ca<sup> 2+</sup>使用棱镜探针和小型荧光显微镜成像自由移动的小鼠

DOI:
10.3791/55579-v

•

10:35 min

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June 13, 2017

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, ,
1Inscopix Inc.

Capítulos

  • 00:05Título
  • 01:12Prism Probe Implant Surgery
  • 04:52Baseplate Attachment for Miniature Microscope Installation
  • 07:22Imaging Multiple Cortical Layers in a Freely Moving Mouse
  • 08:33Results: Calcium Dynamics from Superficial and Deep Layers of Somatosensory Cortex of a Representative Mouse Imaged with the Microscope
  • 09:52Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们提出了一种在自由移动的小鼠中通过多个皮质层进行细胞分辨率的大规模Ca 2+成像的程序。可以使用与植入式棱镜探头耦合的微型头戴式显微镜同时观察数百个活性细胞。

Tags

Keywords: Cortical Calcium ImagingPrism ProbesMiniaturized Fluorescence MicroscopyFreely Moving MiceGenetically Encoded NeuronsMulti-layer Cortical ActivityNeural Circuit FunctionSystems Neuroscience

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