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的多孔冷冻管省去冻结工件时肌肉组织在液氮中直接浸入
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Biologia
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JoVE Journal Biologia
A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen
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的多孔冷冻管省去冻结工件时肌肉组织在液氮中直接浸入

DOI:
10.3791/55616-v

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06:42 min

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April 06, 2017

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, , , , , ,
1State Key Laboratory for Pig Genetic Improvement and Production Technology,Jiangxi Agricultural University

Capítulos

  • 00:05Título
  • 01:17Equipment for Sample Collection and Preparation of Muscle Samples
  • 02:47Freezing and Storing Muscles, and Slide Preparation
  • 04:40Results: Freezing Muscle Tissue Using a Multi-hole Cryovial
  • 06:10Conclusion

Summary

Tadução automática

Tadução automática

这个协议描述的过程通过直接让他们与液氮冷冻的肌肉组织。该协议还突出一个新的离心管，可避免氮气的“毯效应”，当液氮接触样本的组织表面上。

Tags

AI-assisted FreezingCryogenic Sample PreparationMulti-hole CryovialLiquid Nitrogen FreezingFreezing Artifact EliminationCryostat Sectioning

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