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LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics
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Capítulos
- 00:05Título
- 00:44Long-length Anion-exchange (LAX) Capillary Column Packing
- 01:43Tissue Sample Preparation
- 05:33One-dimension Long-length Electrostatic Repulsion-hydrophilic Interaction Chromatography-tandem Mass Spectrometry (LERLIC-MS/MS) Separation and Data Analysis
- 06:48Results: Separation of Deamidation Isoforms of Glutamine and Asparagine by LERLIC-MS/MS
- 07:34Conclusion
Here we present a step-by-step protocol of the long-length electrostatic repulsion-hydrophilic interaction chromatography-tandem mass spectrometry (LERLIC-MS/MS) method. This is a novel methodology that enables for the first time quantification and characterization of the glutamine and asparagine deamidation isoforms by shotgun proteomics.
