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Biologia do Desenvolvimento
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Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

DOI:
10.3791/55652-v

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06:05 min

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May 25, 2017

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1Brain Korea 21 PLUS Project for Medical Science,Yonsei University College of Medicine, 2Department of Anatomy, Brain Research Institute,Yonsei University College of Medicine

Capítulos

  • 00:05Título
  • 00:38Human Plasma Acetylated Low Density Lipoprotein Dil Complex (Dil-AcLDL) Injection Preparation
  • 02:27Dil-AcLDL Injection and Xenopus tropicalis Embryo Fixation
  • 03:44Results: Representative Xenopus tropicalis Imaging
  • 05:02Conclusion

Summary

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This protocol demonstrates a fluorescence-based method to visualize the vasculature and to quantify its complexity in Xenopus tropicalis. Blood vessels can be imaged minutes after the injection of a fluorescent dye into the beating heart of an embryo after genetic and/or pharmacological manipulations to study cardiovascular development in vivo.

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