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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
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Bioquímica
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JoVE Journal Bioquímica
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

DOI:
10.3791/55655-v

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09:29 min

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May 18, 2017

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, , , ,
1Department of Cell Biology and Physiology, School of Medicine,University of North Carolina at Chapel Hill

Capítulos

  • 00:05Título
  • 00:49Preparation of Detergent-soluble and High-salt Extracts of Tissue-specific IFs
  • 03:16Automated Tissue Lysis for RNA and IF Protein Extraction in High-volume Experiments
  • 07:15Results: High-salt Extraction of Intermediate Filament Proteins Using Lysing Matrix
  • 08:50Conclusion

Summary

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In this method, we present biochemical procedures for rapid and efficient isolation of intermediate filament (IF) proteins from multiple mouse tissues. Isolated IFs can be used to study changes in post-translational modifications by mass spectrometry and other biochemical assays.

Tags

Intermediate Filament ProteinsPost-translational ModificationsTriton X-100 BufferHigh Salt BufferPBS EDTA BufferSDS Sample BufferCentrifugationHomogenization

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