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Live-cell Imaging of Platelet Degranulation and Secretion Under Flow
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Biologia
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JoVE Journal Biologia
Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

DOI:
10.3791/55658-v

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11:42 min

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July 10, 2017

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, , , , ,
1Department of Clinical Chemistry and Haematology,University Medical Center Utrecht, 2Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences,Utrecht University

Capítulos

  • 00:05Título
  • 01:12Cover Glass Preparation
  • 02:25Platelet Isolation and Washing
  • 03:58Flow Chamber Assembly
  • 05:08Pre-strained Platelets and Microscope Setup
  • 07:36Microscope Imaging of Platelets
  • 09:31Results: Platelet Degranulation and Secretion Under Flow
  • 10:57Conclusion

Summary

Tadução automática

Tadução automática

This work describes a fluorescence microscopy-based method for the study of platelet adhesion, spreading, and secretion under flow. This versatile platform enables the investigation of platelet function for mechanistic research on thrombosis and hemostasis.

Tags

Keywords: Live-cell ImagingPlatelet DegranulationPlatelet SecretionFlowPlatelet BiologyPlatelet IsolationPlatelet PreactivationVon Willebrand FactorFibrinogenHEPES Tyrode BufferPlatelet-rich PlasmaCitrated Whole Blood

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