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JoVE Journal Biology
Flash-and-Freeze
 
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Flash-and-Freeze: en ny teknikk for å fange Membran Dynamics med elektronmikroskopi

Article DOI: 10.3791/55664-v • 10:01 min • May 1st, 2017
May 1st, 2017 •
*1,2, *1, 1,3
1Department of Cell Biology, Johns Hopkins School of Medicine, 2Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, 3Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
* These authors contributed equally

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Vi har utviklet en ny metode i elektronmikroskopi, "flash-og-fryse", som muliggjør visualisering av membran dynamikk med ms tidsoppløsning. Denne teknikken kombinerer optogenetic stimulering av neuroner med høyt trykk frysing. Her viser vi prosedyrene og beskrive protokoller i detalj.

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Cellular Biology utgave 123 elektronmikroskopi optogenetics synapse membran dynamikk fryse substitusjon channelrhodopsin høytrykks-frysing exocytose endocytose tidsoppløst elektronmikroskopi flash-og-fryse
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