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使用高尔基Cox方法评估老年小鼠海马树突状复杂性
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Neurociência
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JoVE Journal Neurociência
Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method
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使用高尔基Cox方法评估老年小鼠海马树突状复杂性

DOI:
10.3791/55696-v

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09:44 min

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June 22, 2017

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, , ,
1Division of Radiation Health,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 3Neurobiology & Developmental Sciences,University of Arkansas for Medical Sciences

Capítulos

  • 00:05Título
  • 00:55Golgi Staining and Tissue Preparation
  • 02:37Tissue Sectioning
  • 04:33Post-staining Procedures
  • 05:42Mounting, Cleaning, and Covering
  • 07:19Results: Hippocampal Dendritic Complexity in Aged Mice
  • 08:49Conclusion

Summary

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Tadução automática

这里我们详细介绍一种高尔基体Cox协议。这种可靠的组织染色方法允许对海马中的细胞结构和整个大脑进行高质量的评估，同时进行最少的故障排除。

Tags

Golgi-Cox MethodDendritic ComplexityAged MiceNeurociênciaNeuronal StructureGolgi StainingMercuric ChloridePost-impregnation BufferVibratomePBS

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