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Ex 活体人肺肿瘤切片中居民 CD8 T 淋巴细胞的共聚焦显微成像
JoVE Journal
Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
DOI:

08:28 min

December 27, 2017

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Capítulos

  • 00:05Título
  • 01:01Agarose Embedding and Vibratome Slicing of Human Lung Tumors
  • 03:51Immunostaining of Resident CD8 T Cells and Tumor Cells
  • 05:07Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices
  • 07:03Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues
  • 07:53Conclusion

Summary

Tadução automática

本协议描述了一种图像驻留肿瘤浸润 CD8 T 细胞标记的人肺肿瘤切片荧光耦合抗体的方法。这项技术允许 real-time 分析 CD8 T 细胞迁移使用共聚焦显微镜。

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