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Biologia do Desenvolvimento
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通过细胞外囊泡/外来体转移乳腺基质上皮细胞和乳腺腔细胞之间的乳腺形成能力
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes
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通过细胞外囊泡/外来体转移乳腺基质上皮细胞和乳腺腔细胞之间的乳腺形成能力

DOI:
10.3791/55736-v

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09:59 min

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June 03, 2017

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, , , ,
1Institute of Cellular and System Medicine,National Health Research Institutes

Capítulos

  • 00:05Título
  • 01:03Extracellular Vesicle (EV)/Exosome Isolation and Validation and Exosome Density Gradient Purification
  • 03:50Mammary Epithelial Cell EV/Exosome Treatment and Fat Pad Injection
  • 06:20Mammary Gland Whole Mount
  • 07:44Results: Representative Effects of EV/Exosome Mammary Epithelial Cell Treatment
  • 09:29Conclusion

Summary

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Tadução automática

该方案描述了从非贴壁/间充质乳腺上皮细胞中纯化，定量和表征细胞外囊泡（EVs）/外来体的方法，并使用它们将乳腺形成能力转移到乳腺上皮细胞。衍生自茎状乳腺上皮细胞的EVs /外来体可以将该细胞特性转移到摄入EV /外来体的细胞中。

Tags

Extracellular VesiclesExosomesMammary Basal Epithelial CellsMammary Luminal CellsStem Cell PropertiesUltracentrifugationNanoparticle Tracking AnalysisIodixanol Density Gradient

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