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Preparation of Plasma Membrane Vesicles from Bone Marrow Mesenchymal Stem Cells for Potential Cytoplasm Replacement Therapy
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Preparation of Plasma Membrane Vesicles from Bone Marrow Mesenchymal Stem Cells for Potential Cytoplasm Replacement Therapy
DOI:

09:34 min

May 18, 2017

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Capítulos

  • 00:05Título
  • 01:10Plasma Membrane Vesicle (PMV) Generation
  • 03:18PMV Content Examination by Confocal Microscopy
  • 05:11PMV Gastrocnemius Muscle Injection
  • 07:12Results: Representative PMV Imaging and Analyses
  • 08:40Conclusion

Summary

Tadução automática

Age-related diseases are associated with multiple defects in components of the cytoplasm. Here, we present a protocol to prepare plasma membrane vesicles from bone marrow mesenchymal stem cells. This technique could potentially be used as a means of cytoplasm replacement therapy to ameliorate or even reverse age-associated phenotypes.

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