É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1CarMeN Laboratory, INSERM U1060, INRA 1397,University of Lyon, 2Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon,Lyon 1 University, 3Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine,University of Geneva
Capítulos
- 00:05Título
- 00:34Cell Culture Media and Solution Preparation
- 02:45Culture of Human Primary Muscle Cells
- 04:19Insulin Stimulation and Glucose Uptake
- 05:30Cell Lysis
- 06:06Determination of Radiolabeled Glucose
- 06:52Results: Effective Glucose Uptake Measurement and Analysis of Response to Insulin Stimulation
- 07:37Conclusion
Bei diesem Verfahren werden menschliche primäre Muskelzellen in vitro kultiviert, um differenzierte Myotubes zu erhalten, und Glucose-Aufnahmeraten werden gemessen. Wir liefern ein detailliertes Protokoll zur Quantifizierung von Raten in basalen und insulin-stimulierten Zuständen unter Verwendung von radioaktiv markiertem [ 3 H] 2-Desoxy-D-Glucose.
