Journal
/
Genética
/
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

DOI:
10.3791/55745-v

•

12:02 min

•

June 06, 2017

•

, ,
1Department of Cell Biology and Histology,University of the Basque Country, UPV/EHU, 2Department of Genetics, Physical Anthropology and Animal Physiology,University of the Basque Country, UPV/EHU, 3Department of Molecular Mechanisms of Disease,University of Zurich

Capítulos

  • 00:05Título
  • 00:41Thymidine- and Nocodazole-based (Thy-noc) Synchronization and Release
  • 03:34HU-based Synchronization and Release from G1/S Boundary
  • 05:14Collecting Samples for FACS Analysis and RNA Extraction
  • 07:00RNA Extraction for Gene Expression Analysis
  • 09:02Results: Thy-noc and HU Cell Synchronization Protocols Enable Analysis of Gene Expression During Specific Phases of the Cell Cycle
  • 11:07Conclusion

Summary

Tadução automática

Tadução automática

We report two cell synchronization protocols that provide a context for studying events related to specific phases of the cell cycle. We show that this approach is useful for analyzing the regulation of specific genes in an unperturbed cell cycle or upon exposure to agents affecting the cell cycle.

Tags

Keywords: Cell CycleGene ExpressionCell SynchronizationU2OS CellsThymidine BlockNocodazoleMitotic Cell ArrestG2-MRNA ExtractionFACS Analysis

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/55745?article_type=v

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
check_url/pt/55745?article_type=v

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
check_url/pt/55745?article_type=v

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
check_url/pt/55745?article_type=v

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
check_url/pt/55745?article_type=v

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
check_url/pt/55745?article_type=v

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
check_url/pt/55745?article_type=v

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans
check_url/pt/55745?article_type=v

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
check_url/pt/55745?article_type=v

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
check_url/pt/55745?article_type=v

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Read Article