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通过两个互补细胞同步方案研究细胞周期调控的基因表达

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Genética

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JoVE Journal Genética

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

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通过两个互补细胞同步方案研究细胞周期调控的基因表达

DOI:

10.3791/55745-v

•

12:02 min

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June 06, 2017

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Aintzane Apraiz*¹, Jone Mitxelena*^2,3, Ana Zubiaga

¹Department of Cell Biology and Histology,University of the Basque Country, UPV/EHU, ²Department of Genetics, Physical Anthropology and Animal Physiology,University of the Basque Country, UPV/EHU, ³Department of Molecular Mechanisms of Disease,University of Zurich

Capítulos

00:05Título
00:41Thymidine- and Nocodazole-based (Thy-noc) Synchronization and Release
03:34HU-based Synchronization and Release from G1/S Boundary
05:14Collecting Samples for FACS Analysis and RNA Extraction
07:00RNA Extraction for Gene Expression Analysis
09:02Results: Thy-noc and HU Cell Synchronization Protocols Enable Analysis of Gene Expression During Specific Phases of the Cell Cycle
11:07Conclusion

Summary

Tadução automática

我们报告两个细胞同步协议，提供一个上下文，用于研究与细胞周期的特定阶段相关的事件。我们表明，这种方法可用于分析未受扰动细胞周期中特定基因的调节，或暴露于影响细胞周期的试剂。

Tags

Keywords: Cell Cycle Gene Expression Cell Synchronization U2OS Cells Thymidine Block Nocodazole Mitotic Cell Arrest G2-M RNA Extraction FACS Analysis

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