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Biologia do Desenvolvimento

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在体外人骨髓间充质干细胞分化为心肌样细胞功能

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

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在体外人骨髓间充质干细胞分化为心肌样细胞功能

DOI:

10.3791/55757-v

•

09:05 min

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August 09, 2017

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Peter Szaraz*^1,2, Yarden S. Gratch*¹, Farwah Iqbal², Clifford L. Librach^2,3,4,5

¹Create Fertility Centre, ²Department of Physiology,University of Toronto, ³Department of Obstetrics and Gynecology,University of Toronto, ⁴Department of Physiology,University of Toronto, ⁵Department of Obstetrics and Gynecology,Women’s College Hospital

Capítulos

00:05Título
00:58Preparation of Primary Rat Cardiac, Human MSC Co-cultures
03:55Preparation of Aggregate Co-cultures
05:43Results: Differentiation of Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
07:53Conclusion

Summary

Tadução automática

在这里，我们提出一种方法，有效地利用的心肌细胞分化潜力，年轻的人骨髓间充质干细胞的来源以生成功能、订约、心肌样细胞体外。

Tags

Keywords: Mesenchymal Stem Cells Cardiomyocyte Differentiation In Vitro Differentiation Cardiac Fetal Layers Cardiovascular Disease Trypsin Digestion DMEM-F12 MSC Cultures Cell Dissociation

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