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蛋白质 - 蛋白质相互作用的分析和鼠乳腺中间隙，紧密和粘附连接部位之间的共定位
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
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蛋白质 - 蛋白质相互作用的分析和鼠乳腺中间隙，紧密和粘附连接部位之间的共定位

DOI:
10.3791/55772-v

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11:31 min

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May 30, 2017

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,
1INRS, Institut Armand-Frappier, 2Biomed, UQAM

Capítulos

  • 00:05Título
  • 00:48Identifying Proetin Co-localization
  • 03:41Studying Protein-protein Interactions (PPIs): Indirect Immunoprecipitation
  • 06:29Downstream Application: Gel Electrophoresis Followed by Western Blot
  • 08:26Results: Interaction of Gap Junction, Adhered Junction, and Tight Junction Components in the Murine Mammary Gland
  • 10:21Conclusion

Summary

Tadução automática

Tadução automática

细胞间连接是乳腺阶段特异性功能和发育的必需条件。该手稿提供了一个详细的方法，用于研究蛋白质 - 蛋白质相互作用（PPI）和使用鼠乳腺的共定位。这些技术允许调查不同发育阶段的细胞间连接之间的物理联系的动力学。

Tags

Protein-protein InteractionsCo-localizationIntercellular JunctionsMammary GlandsCo-immunoprecipitationCo-immunofluorescent StainingFormaldehyde FixationPhosphate-buffered SalineBovine Serum AlbuminTris-Buffered SalinePolysorbate-20Primary AntibodySecondary AntibodyFluorescent Conjugation

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