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微型固相萃取和LC-MS / MS检测人尿中3-硝基酪氨酸的临床应用
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JoVE Journal Química
Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
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微型固相萃取和LC-MS / MS检测人尿中3-硝基酪氨酸的临床应用

DOI:
10.3791/55778-v

•

08:41 min

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July 14, 2017

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, , ,
1Pharmasan Labs, Inc., 2NeuroScience, Inc.

Capítulos

  • 00:05Título
  • 01:41Solid Phase Extraction Procedure
  • 05:00LC-MS/MS Analysis
  • 06:17Results: LC-MS/MS Determination of 3-Nitrotyrosine in Human Urine
  • 07:47Conclusion

Summary

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Tadução automática

开发了选择性和灵敏的液相色谱串联质谱（LC-MS / MS）方法与混合模式阳离子交换（MCX）96孔微量培养板上的有效固相萃取相结合，用于测量游离的3-硝基酪氨酸（ 3-NT），具有高通量，适用于临床应用。

Tags

3-nitrotyrosineUrineSolid Phase ExtractionLC-MS/MSOxidative StressBiomarkerClinical Application

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