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Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method

Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method

DOI:
10.3791/55789-v

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11:02 min

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July 12, 2017

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,
1National Toxicology Program Laboratory, Division of the National Toxicology Program,National Institute of Environmental Health Sciences

Capítulos

  • 00:05Título
  • 00:41Preparing the Whole-mount Mammary Gland Image
  • 05:48The Sholl Analysis
  • 08:15Measuring the Mammary Epithelial Area (MEA)
  • 09:05Results: Interpreting the Data
  • 10:23Conclusion

Summary

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Mammary gland development in the rodent has typically been evaluated using descriptive assessments or by measuring basic physical attributes. Branching density is an indicator of mammary development that is difficult to quantify objectively. This protocol describes a reliable method for the quantitative assessment of mammary gland branching characteristics.

Tags

Keywords: Mammary GlandBranching DensityBranching DecaySholl AnalysisComputer-assisted MethodWhole-mount ImagesImageJGlandular EpitheliumLymph NodeColor ChannelsBackground SubtractionUnsharp MaskNoise Removal

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