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Biologia do Desenvolvimento
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Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

DOI:
10.3791/55792-v

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12:38 min

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September 20, 2017

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, , , ,
1Department of Biological Sciences,University of Maryland, Baltimore County

Capítulos

  • 00:05Título
  • 00:45Fixation of Staged Embryos
  • 01:52Sectioning for Immunolabeling
  • 04:04Confocal Imaging
  • 05:17Quantifying Microtubules
  • 07:10De Novo MT Assembly Assay
  • 09:05Depolymerize Existing MTs
  • 10:26Results: Analysis of Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
  • 12:00Conclusion

Summary

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Tadução automática

Immunolabeling methods to analyze distinct populations of microtubules in the developing zebrafish brain are described here, which are broadly applicable to other tissues. The first protocol outlines an optimized method for immunolabeling stable and dynamic microtubules. The second protocol provides a method to image and quantify nascent microtubules specifically.

Tags

ImmunolabelingMicrotubulesZebrafish EmbryoDevelopmental NeurobiologyCell PolarityStable MicrotubulesDynamic MicrotubulesNascent MicrotubulesFixationSectioningImmunostainingPrimary AntibodiesSecondary AntibodiesDAPI

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