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Microcrystallography of Protein Crystals and In Cellulo Diffraction
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Bioquímica
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JoVE Journal Bioquímica
Microcrystallography of Protein Crystals and In Cellulo Diffraction

Microcrystallography of Protein Crystals and In Cellulo Diffraction

DOI:
10.3791/55793-v

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09:35 min

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July 21, 2017

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, ,
1Infection and Immunity Program, Monash Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology,Monash University

Capítulos

  • 00:05Título
  • 01:12Identification of Crystal-containing Cells
  • 02:46Purification of Crystal-containing Cells
  • 05:00Sample Preparation for Data Collection
  • 06:47Data Collection
  • 07:42Resultados
  • 08:53Conclusion

Summary

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A protocol is presented for X-ray crystallography using protein microcrystals. Two examples analyzing in vivo-grown microcrystals after purification or in cellulo are compared.

Tags

Keywords: MicrocrystallographyProtein CrystalsIn Cellulo DiffractionX-ray CrystallographyMicrocrystalsIn Vivo CrystalsSf9 CellsCypovirus Polyhedron ProteinFlow CytometryPropidium Iodide

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