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骨髓衍生祖细胞的低氧预处理作为成熟雪旺细胞产生的来源

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells

Please note that all translations are automatically generated. Click here for the English version.

骨髓衍生祖细胞的低氧预处理作为成熟雪旺细胞产生的来源

DOI:

10.3791/55794-v

•

10:16 min

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June 14, 2017

•

Yat-Ping Tsui*¹, Alan Kwan-Long Mung*¹, Ying-Shing Chan, Daisy Kwok-Yan Shum, Graham Ka-Hon Shea

¹School of Biomedical Sciences,The University of Hong Kong, ²Department of Orthopaedics and Traumatology,The University of Hong Kong

Capítulos

00:05Título
00:47MSC Culture Establishment and Expansion
02:13Schwann Cell-like Cell (SCLC) Generation
04:40Dorsal Root Ganglion (DRG) Purification and SCLC Co-culture
07:44Results: Representative Schwann Cell Generation
09:08Conclusion

Summary

Tadução automática

具有神经电位的骨髓基质细胞（MSC）存在于骨髓内。我们的方案通过缺氧预处理丰富了这种细胞群，之后引导他们成为成熟的雪旺氏细胞。

Tags

Keywords: Hypoxic Preconditioning Marrow-derived Progenitor Cells Schwann Cells Stem Cell Regenerative Medicine Neural Progenitors Bone Marrow Stromal Cells Autologous Cell Therapy MSC Growth Medium Cell Dissociation Hypoxia Chamber Hepes Nitrogen Oxygen

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