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Biologia do Desenvolvimento

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新型乳腺脂肪移植技术可视化血管内皮干细胞的血管生成

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells

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新型乳腺脂肪移植技术可视化血管内皮干细胞的血管生成

DOI:

10.3791/55795-v

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12:41 min

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August 03, 2017

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Qing Cissy Yu, Wenqian Song, Dengwen Lai, Yi Arial Zeng

¹State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences

Capítulos

00:05Título
01:26Isolation of VESCs from the Mouse Mammary Gland
04:42In Vivo VESC Vessel Formation Assay Using the Mammay Fat Pad
08:05Whole Mount Tissue Preparation for Microscopic Observation
10:23Results: Isolation of Vascular Endothelial Stem Cells and Vessel Generation of Tranplanted Cells
11:27Conclusion

Summary

Tadução automática

这项工作展示了一种新的方法来评估通过乳腺脂肪移植移植血管内皮细胞（VESCs）的增殖，分化和血管形成潜力，然后进行全面的组织制备用于显微镜观察。还提出了研究VESCs 体内行为的谱系追踪策略。

Tags

Keywords: Mammary Fat Pad Vascular Endothelial Stem Cells Vessel Generation Transplantation Technique Angiogenic Environment Cell Isolation Microscopic Observation

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