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Biologia do Desenvolvimento
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Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

DOI:
10.3791/55818-v

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08:28 min

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June 30, 2017

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, , ,
1Department of Large Animal Clinical Sciences,Michigan State University, 2Department of Pharmacology and Toxicology,Michigan State University

Capítulos

  • 00:05Título
  • 00:50Adipocyte Progenitors Isolation
  • 02:52Magnetic Activated Cell Sorting
  • 05:13Cell Culture and Adipogenesis Induction
  • 06:35Results: Representative Adipocyte Progenitor Cells (APCs) Differentiation Analysis
  • 07:57Conclusion

Summary

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Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).

Tags

Adipocyte Progenitor CellsMagnetic Activated Cell SortingAdipogenesisStromal Vascular FractionCollagenase DigestionCell FiltrationErythrocyte Lysis

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