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Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse
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JoVE Journal Medicina
Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse

Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse

DOI:
10.3791/55843-v

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10:22 min

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October 26, 2017

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, , , ,
1Université Côte d’Azur,CNRS, INSERM, iBV, 2Department of Pathology,CHU Nice, 3Institute for Molecular Health Sciences,ETH Zurich

Capítulos

  • 00:05Título
  • 01:18Echocardiography
  • 03:25Image Analysis
  • 05:59Preparation of Heart Samples for Histological Evaluation and WGA Staining
  • 07:50Results: Cardiac Morphology Analysis Using Echocardiography and Histology
  • 09:12Conclusion

Summary

Tadução automática

Tadução automática

Echocardiographic examination is frequently used in mice. Expensive high-resolution ultrasound devices have been developed for this purpose. This protocol describes an affordable echocardiographic procedure combined with histological morphometric analyses to determine cardiac morphology.

Tags

Keywords: EchocardiographyCardiac MorphologyMouseTransgenic ModelsAnesthesiaBody WeightShavingParasternal ViewM-mode ImagingLeft Ventricular DimensionsSystoleDiastoleWall ThicknessHistological Examination

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