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Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
JoVE Journal
Química
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JoVE Journal Química
Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
DOI:

11:08 min

February 05, 2018

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Capítulos

  • 00:04Título
  • 01:23Preparation of Glycans with Phosphonic Acid Tail
  • 04:12Glycan Array
  • 08:18Results: Synthesis of N-glycans and Development of Arrays for HIV Antibody Profiling
  • 09:59Conclusion

Summary

Tadução automática

A modular approach to the synthesis of N-glycans for attachment to an aluminum oxide-coated glass slide (ACG slide) as a glycan microarray has been developed and its use for the profiling of an HIV broadly neutralizing antibody has been demonstrated.

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