Journal
/
Neurociência
/
Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

DOI:
10.3791/55862-v

•

07:30 min

•

September 04, 2017

•

, ,
1National Institute of Neurological Disorders and Stroke, 2College of Pharmacy,Chung-ang University

Capítulos

  • 00:05Título
  • 00:40Culture Stimulation
  • 01:35Fixation and Staining
  • 03:42Epoxy Embedding
  • 05:43Results: Images of Presynaptic Terminal in Stimulated Neurons with High Potassium
  • 06:49Conclusion

Summary

Tadução automática

Tadução automática

Synaptic vesicle endocytosis is detected by light microscopy of pHluorin fused with synaptic vesicle protein and by electron microscopy of vesicle uptake.

Tags

Synaptic Vesicle EndocytosisHippocampal NeuronsPoly-d-lysineHigh Potassium StimulationGlutaraldehydeSodium Cacodylate BufferDAB SolutionOsmium TetroxideSodium Acetate BufferUranyl AcetateDehydrationElectron Microscopy

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/55862?article_type=v

Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes
check_url/pt/55862?article_type=v

Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
check_url/pt/55862?article_type=v

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
check_url/pt/55862?article_type=v

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes
check_url/pt/55862?article_type=v

Inducing Dendritic Growth in Cultured Sympathetic Neurons
check_url/pt/55862?article_type=v

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
check_url/pt/55862?article_type=v

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)
check_url/pt/55862?article_type=v

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
check_url/pt/55862?article_type=v

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
check_url/pt/55862?article_type=v

Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities

Read Article