Journal
/
Bioquímica
/
Quantitative Immunofluorescence to Measure Global Localized Translation
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Quantitative Immunofluorescence to Measure Global Localized Translation

Quantitative Immunofluorescence to Measure Global Localized Translation

DOI:
10.3791/55909-v

•

09:13 min

•

August 22, 2017

•

,
1Centre de Recherche sur le Cancer de l’Université Laval, Faculté de Médecine, Département de Biologie moléculaire, biochimie médicale et pathologie,Université Laval, 2CRCHU de Québec: L’Hôtel-Dieu de Québec

Capítulos

  • 00:05Título
  • 00:45Cell Immunostaining
  • 03:34Image Acquisition
  • 04:49Signal Quantification
  • 06:50Results: Proper Puromycin Treatment and Analysis in MRC-5 and Other Cell Types
  • 08:16Conclusion

Summary

Tadução automática

Tadução automática

This manuscript describes a method to visualize and quantify localized translation events in subcellular compartments. The approach proposed in this manuscript requires a basic confocal imaging system and reagents and is rapid and cost-effective.

Tags

Keywords: Quantitative ImmunofluorescenceLocalized TranslationCell AdhesionPuromycinAnti-puromycin AntibodyFluorophore-conjugated Secondary AntibodiesCell MigrationCell AdhesionEarly Drug ResponseMRC-5 CellsCycloheximideFormaldehyde FixationTriton X-100 PermeabilizationBSA BlockingTween-20 Washing

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/55909?article_type=v

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
check_url/pt/55909?article_type=v

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs
check_url/pt/55909?article_type=v

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
check_url/pt/55909?article_type=v

Ubiquitin Chain Analysis by Parallel Reaction Monitoring
check_url/pt/55909?article_type=v

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
check_url/pt/55909?article_type=v

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
check_url/pt/55909?article_type=v

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
check_url/pt/55909?article_type=v

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
check_url/pt/55909?article_type=v

Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
check_url/pt/55909?article_type=v

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases

Read Article