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全自动毛细 Electrophoretic-based 免疫分析方法的程序和关键优化策略

JoVE Journal
Biologia
Author Produced

This content is Free Access.

JoVE Journal Biologia

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

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全自动毛细 Electrophoretic-based 免疫分析方法的程序和关键优化策略

DOI:

10.3791/55911-v

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09:32 min

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September 10, 2017

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Gail M Nelson, Jenna M Guynn, Brian N Chorley

¹National Health and Environmental Effects Research Laboratory,U.S. Environmental Protection Agency, ²Oak Ridge Institute for Science and Education at U.S. Environmental Protection Agency

Summary

Tadução automática

利用商用平台检测总蛋白制剂中靶蛋白的 capillary-based 免疫分析方法。另外, 对细胞培养模型系统进行了暴露时间、蛋白质浓度和抗体稀释度的测定参数的优化。

Tags

Keywords: Capillary Electrophoresis Immunoassay Automated Procedure Protein Quantitation Cell Culture Biofluids Western Blot Sample Preparation Master Mix Denaturing Conditions Antibody Dilutions

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