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Real-time Tracking of DNA Fragment Separation by Smartphone
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JoVE Journal Bioquímica
Real-time Tracking of DNA Fragment Separation by Smartphone

Real-time Tracking of DNA Fragment Separation by Smartphone

DOI:
10.3791/55926-v

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06:58 min

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June 01, 2017

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, , , ,
1Shanghai Key Lab of Modern Optical System,University of Shanghai for Science and Technology, 2Department of Applied Physics, Graduate School of Engineering,Osaka University, 3East China University of Science and Technology,Department of Physics Faculty of Science

Capítulos

  • 00:00Título
  • 01:05Preparation of the Agarose Gel for the Chip
  • 02:13Separation of DNA Fragments in the SGE Chip
  • 05:45Results: Agarose Gel Electrophoresis of DNA Fragments
  • 06:15Conclusion

Summary

Tadução automática

Tadução automática

Traditional slab gel electrophoresis (SGE) experiments require a complicated apparatus and high chemical consumption. This work presents a protocol that describes a low-cost method to separate DNA fragments within a short timeframe.

Tags

Keywords: DNA Fragment SeparationSNAP Gel ElectrophoresisReal-time TrackingSmartphoneAgarose GelDNA LadderSYBR GreenLED Light SourceBand-pass FilterElectrodePower SourceBiochemical AnalysisBiological Analysis

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