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Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
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JoVE Journal Medicina
Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

DOI:
10.3791/55942-v

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07:05 min

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December 15, 2017

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, , , , , , ,
1Department of Medicine B,University Hospital Münster, 2Translational Research Imaging Center, Department of Clinical Radiology,University Hospital Münster

Capítulos

  • 00:05Título
  • 00:51Fluorescence-mediated Tomography (FMT)
  • 03:33Data Reconstruction and Interpretation
  • 04:47Results: Representative Fluorescence-mediated Tomography
  • 06:17Conclusion

Summary

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Tadução automática

Target-specific probes represent an innovative tool for analyzing molecular mechanisms, such as protein expression in various types of disease (e.g., inflammation, infection, and tumorigenesis). In this study, we describe a quantitative three-dimensional tomographic assessment of intestinal macrophage infiltration in a murine model of colitis using F4/80-specific fluorescence-mediated tomography.

Tags

Fluorescence-mediated TomographyMacrophageIntestinal InflammationInflammatory Bowel DiseaseIn Vivo ImagingDextran Sodium SulfateAntibodyFluorescence-mediated Tomography DeviceTracerAnesthetized MouseFluorescence Reflectance Image

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