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使用体内和组织和细胞外植体方法研究胚胎和围产期主动脉的形态和发病机制
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
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使用体内和组织和细胞外植体方法研究胚胎和围产期主动脉的形态和发病机制

DOI:
10.3791/56039-v

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10:57 min

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September 12, 2017

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, , , ,
1Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Department of Internal Medicine,Yale University School of Medicine, 2Department of Neurology,Yale University School of Medicine, 3Department of Neurology, Xinhua Hospital,Shanghai Jiaotong University School of Medicine

Capítulos

  • 00:05Título
  • 01:07In Vivo Embryonic Fate Mapping and Clonal Analysis
  • 04:14Explant Embryonic Aorta Culture
  • 05:47Smooth Muscle Cell (SMC) Perinatal Aorta Isolation
  • 07:16Subcutaneous Osmotic Mini-Pump Placement
  • 08:23Results: Representative Embryonic and Neonatal SMC Analyses
  • 09:47Conclusion

Summary

Tadução automática

Tadução automática

用于研究胚胎和围产期小鼠主动脉的协议使用在体内克隆分析和命运图, 主动脉外植体和孤立平滑肌细胞在这里详述。这些不同的方法有助于调查胚胎和围产期主动脉在正常发育和疾病的发病机制。

Tags

AortaVascular DevelopmentSmooth MuscleSupravalvular Aortic StenosisEmbryonicPerinatalMorphogenesisPathogenesisIn VivoCell ExplantCre ERTamoxifenProgesteroneParaformaldehydeSucroseOCT CompoundCryosectioning

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