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A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
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Biologia
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JoVE Journal Biologia
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

DOI:
10.3791/56053-v

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07:54 min

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July 25, 2017

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, , , ,
1Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA),Consejo Superior de Investigaciones Científicas (CSIC), 2Department of Food Safety and Infection Biology,Norwegian University of Life Sciences (NMBU), 3Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria,Universidad Complutense de Madrid (UCM), 4Faculty of Chemistry, Biotechnology and Food Science,Norwegian University of Life Sciences (NMBU)

Capítulos

  • 00:05Título
  • 00:40Preparation of Bacterial Cultures to Inoculate Mice
  • 01:46Mice Assay and Experimental Design and Collecting Samples
  • 03:08LAB Counting and Bacteriocin Activity
  • 04:39DNA Extraction, 16S rDNA Amplification, and Sequencing
  • 05:45Results: Detection and Comparison of Bacteriocin Production in Lactic Acid Bacteria Recovered from Feces
  • 07:12Conclusion

Summary

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Bacteriocins are believed to play a key role in defining microbial diversity in different ecological niches. Here, we describe an efficient procedure to assess how bacteriocins affect gut microbiota composition in an animal model.

Tags

Gut MicrobiotaBacteriocinsMouse ModelProbioticBacterial PopulationsPhysiological ParametersHost OrganismSerial DilutionsColony Forming UnitsDrinking WaterBacterial SurvivalBALB/c MiceFecal SamplesBacterial SuspensionBacteriocin-producing BacteriaMRS Soft Agar

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