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Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
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Capítulos
- 00:05Título
- 00:49Protocol 1: Generation of Escape Mutant Variants Against HI-positive/Neutralization-positive Antibodies
- 02:51Protocol 2: Generation of Escape Mutant Variants Against HI-negative/Neutralization-positive Antibodies
- 06:17Results: Epitope Map of the Novel Avian H7N9 HA Generated with Escape Mutant Variants
- 07:11Conclusion
We describe a method by which we identify critical residues required for the binding of human or murine monoclonal antibodies that target the viral hemagglutinin of influenza A viruses. The protocol can be adapted to other virus surface glycoproteins and their corresponding neutralizing antibodies.
