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Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling
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JoVE Journal Medicina
Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling

Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling

DOI:
10.3791/56075-v

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09:51 min

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July 26, 2017

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, , , ,
1Department of Respiratory Medicine, Shanghai First People’s Hospital,Shanghai Jiaotong University School of Medicine, 2Department of Pediatrics,BC Children’s Hospital and University of British Columbia

Capítulos

  • 00:05Título
  • 00:57Preparation of Cell Culture Media and Reagents
  • 02:10Culture of THP-1 Reporter Cell-derived Macrophages
  • 03:45Screening for Potential TLR4 Nano-inhibitors Using the Reprter Cells
  • 07:22Results: Inhibition of Toll-like Receptor Signaling
  • 09:13Conclusion

Summary

Tadução automática

Tadução automática

Toll-like receptor (TLR) signaling plays an important role in the pathophysiology of many human inflammatory diseases, and regulating TLR responses by bioactive nanoparticles is anticipated to be beneficial in many inflammatory conditions. THP-1 cell-based reporter cells provide a versatile and robust screening platform for identifying novel inhibitors of TLR signaling.

Tags

Keywords: Bioactive NanoparticlesToll-like Receptor SignalingInnate Immune CellsInflammatory DiseasesPhagocytic Immune CellsReporter Cell SystemsNanoinhibitorsScreening ProcedureLuciferase SubstrateSEAP SubstrateCell CulturePMA

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