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用可寻址滴微阵列计算单细胞中的蛋白质

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Counting Proteins in Single Cells with Addressable Droplet Microarrays

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用可寻址滴微阵列计算单细胞中的蛋白质

DOI:

10.3791/56110-v

•

12:25 min

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July 06, 2018

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Stelios Chatzimichail*¹, Pashiini Supramaniam*¹, Oscar Ces, Ali Salehi-Reyhani

¹Institute of Chemical Biology, Department of Chemistry,Imperial College London

Capítulos

00:05Título
00:55Prepare Apparatus for Dispensing Addressable Droplets
03:26Form Addressable Droplets and Load with Single Cells
07:42Data Analysis
09:39Results: Single Cell Data
11:08Conclusion

Summary

Tadução automática

在这里, 我们提出了可寻址的液滴微阵列 (ADMs), 一种能够确定单细胞中绝对蛋白质丰度的液滴数组方法。我们展示了 ADMs 的能力来表征肿瘤抑制蛋白 p53 在人类癌细胞系中表达的异质性。

Tags

Single Cell Protein Copy Number Addressable Droplet Microarray Heterogeneity Central Dogma Gene Expression Fused Silica Tubing Concentric Nozzle PTFE Tubing FEP Tubing Hamilton Syringe Cyanoacrylate Glue PBSA Blocking Solution

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