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Cryopreservatie van zebravis testis van hele Testes Needle ondergedompeld Ultra snelle koeling
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Biologia
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JoVE Journal Biologia
Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
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Cryopreservatie van zebravis testis van hele Testes Needle ondergedompeld Ultra snelle koeling

DOI:
10.3791/56118-v

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13:32 min

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March 04, 2018

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, , , , ,
1Department of Aquaculture,Szent István University

Capítulos

  • 00:00Título
  • 01:14Testis collection
  • 03:29Ultra-rapid cooling of the testicular tissue
  • 06:11Warming procedure
  • 07:56Tissue digestion
  • 10:27Viability evaluation and cell counting
  • 11:28Representative Results
  • 12:20Conclusions

Summary

Tadução automática

Tadução automática

Het belangrijkste doel van deze studie was aan te passen de naald ondergedompeld verglazing (NIV) procedure om te cryopreserve hele zebrafish teelballen. Daarnaast is de herhaalbaarheid van de methode in vijf verschillende zebrafish stammen was getest.

Tags

CryopreservationZebrafishSpermatogoniaVitrificationWhole TestesUltra-rapid CoolingGerm CellsTransplantationSlow-rate FreezingSpermatozoaEuthanasiaDissectionTestes RemovalSterilizationEquilibrationVitrification Solutions

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