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Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
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Capítulos
- 00:05Título
- 01:13Adult Brain Dissection
- 03:39Brain Fixation and Immunofluorescent Staining
- 06:09Mounting Adult D. melanogaster Brains onto Microscope Slides and Imaging
- 07:47Results: Analyzing Axon Guidance Defects with the Mushroom Bodies
- 09:04Conclusion
This protocol describes the dissection and immunostaining of adult Drosophila melanogaster brain tissues. Specifically, this protocol highlights the use of Drosophila mushroom body and photoreceptor neurons as example neuronal subsets that can be accurately used to uncover general principles underlying many aspects of neuronal development.
