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Biologia
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Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles
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Biologia
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JoVE Journal Biologia
Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

DOI:
10.3791/56179-v

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06:43 min

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March 31, 2018

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, ,
1Department of Genetics and Interdisciplinary Research Structure for Biotechnology and Biomedicine (BIOTECMED),University of Valencia, 2Incliva Health Research Institute, 3Joint unit CIPF-Incliva

Capítulos

  • 00:00Título
  • 00:32Fixation and resin embedding
  • 02:53Trimming and sectioning of blocks
  • 03:45Staining IFM sections
  • 04:06Mounting IFM sections
  • 04:26Acquisition of images and quantification of muscle area
  • 05:10Representative results
  • 06:05Conclusions

Summary

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We report a method to quantify muscle area, which is an indirect method to determine muscle mass in Drosophila adults. We demonstrate the application of our methodology by analyzing the indirect flight muscles in a Drosophila model of Myotonic Dystrophy disease.

Tags

Drosophila MelanogasterOptical Cross-sectional Muscle AreaIndirect Flight MusclesMuscle MassMuscle AtrophyDorsal Longitudinal MusclesFixationResin EmbeddingAnesthesiaDehydrationPropylene OxideResin PolymerizationTrimmingSectioningUltramicrotome

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