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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
JoVE Journal
Genética
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JoVE Journal Genética
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

DOI:
10.3791/56221-v

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09:04 min

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September 21, 2017

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, ,
1Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences,Nanyang Technological University

Capítulos

  • 00:05Título
  • 00:45Non-denaturing PAGE
  • 02:342-Aminopurine Fluorescence Binding Assay
  • 05:16UV-absorbance-detected Thermal Melting Experiments
  • 06:36Results: Characterization of Binding Properties of Chemically Modified Peptide Nucleic Acids
  • 08:18Conclusion

Summary

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We report the protocols for the synthesis and purification of Peptide Nucleic Acid (PNA) oligomers incorporating modified residues. The biochemical and biophysical methods for the characterization of the recognition of RNA duplexes by the modified PNAs are described.

Tags

Keywords: RNAPNADouble-stranded RNASingle-stranded RNAMolecular RecognitionChemical BiologyRNA StructureGel Electrophoresis2-aminopurineAnnealingIncubation BufferVacuum Concentrator

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